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	Provedor de dados Electron. J. Biotechnol. (2) Autor Altamirano,Claudia (2) Becerra,Silvana (2) Berrios,Julio (2) Vergara,Mauricio (2) Díaz-Barrera,Alvaro (1) Palavra-chave CHO cells (2) Aerobic glycolysis (1) Cell engineering (1) Galactose (1) Glutamate (1) Glutaminolysis (1) Low temperature (1) Metabolism (1) Perfusion culture (1) Rh-tPA (1) Mais... Tipo do documento Journal article (2) Ano 2013 (1) 2012 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Advances in improving mammalian cells metabolism for recombinant protein production Electron. J. Biotechnol. Altamirano,Claudia; Berrios,Julio; Vergara,Mauricio; Becerra,Silvana. Background: The production of recombinant proteins for therapeutic use represents a great impact on the biotechnology industry. In this context, established mammalian cell lines, especially CHO cells, have become a standard system for the production of such proteins. Their ability to properly configure and excrete proteins in functional form is an enormous advantage which should be contrasted with their inherent technological limitations. These cell systems exhibit a metabolic behaviour associated with elevated cell proliferation which involves a high consumption of glucose and glutamine, resulting in the rapid depletion of these nutrients in the medium and the accumulation of ammonium and lactate. Both phenomena contribute to the limitation of cell... Tipo: Journal article Palavras-chave: Aerobic glycolysis; Cell engineering; CHO cells; Glutaminolysis; Metabolism. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300010 Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA Electron. J. Biotechnol. Vergara,Mauricio; Becerra,Silvana; Díaz-Barrera,Alvaro; Berrios,Julio; Altamirano,Claudia. We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamster ovary cells in batch and semi-perfusion cultures. In batch cultures using glucose as a carbon source, decreasing the temperature caused a reduction in cell growth and an increase in specific productivity of rh-tPA of 32% at 34ºC and 55% at 31ºC, compared to cultures at 37ºC. Similar behaviour was observed in cultures at 34ºC using galactose as a carbon source. Nonetheless, at 31ºC, the specific productivity of rh-tPA strongly decreased (about 58%) compared to... Tipo: Journal article Palavras-chave: CHO cells; Galactose; Glutamate; Low temperature; Perfusion culture; Rh-tPA. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600002 Registros recuperados: 2 Primeira ... 1 ... Última

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